Promega UK Launches Lumit Cell Proliferation Assay for Simplified hKi-67 Detection

Problem: Inefficient and Time-Consuming Cell Proliferation Analysis

Cell proliferation assessment is a critical component of various research workflows, including oncology drug screening, immunology, and cell therapy applications. Traditional methods for detecting the proliferation marker hKi-67 rely on fixation-based flow cytometry, which involves laborious and time-consuming steps such as washing, fixation, and dissociation. These methods typically require treatment windows of 72 to 96 hours and introduce variability due to multiple handling steps, making them less than ideal for researchers seeking reliable and rapid results.

Solution: A Homogeneous, Wash-Free Assay Using Lumit Technology

Promega UK has introduced the Lumit Cell Proliferation Assay (Human Ki-67), a homogeneous, wash-free assay designed to streamline the detection of hKi-67. This assay utilizes Lumit technology, which employs labeled primary antibodies that form a fully functional NanoBiT luciferase enzyme in the presence of hKi-67. The addition of a substrate generates a luminescent signal proportional to Ki-67 levels, eliminating the need for fixation, dissociation, or washing steps.

Key features of the assay include: - **Simplicity**: A straightforward add-and-read format that reduces preparation time and minimizes variability. - **Versatility**: Compatible with both 96- and 384-well microplate formats, as well as 3D culture methods, making it adaptable to a wide range of experimental workflows. - **Performance**: Offers excellent linearity and a robust response, enabling researchers to generate more data with greater confidence.

"Cell proliferation is a fundamental parameter assessed across a wide range of cell-based research workflows... Our new Lumit Cell Proliferation Assay addresses this directly – tracking hKi-67 in a simple add-and-read format that delivers results faster and with greater confidence." - Philip Hargreaves, Director of Strategic Marketing and Business Development, Promega UK

Results: Faster, More Reliable Data Generation

The Lumit Cell Proliferation Assay has been validated by researchers such as Zeynep Kaya, PhD, from the University of Birmingham, who noted that the assay is "significantly simpler and faster" than conventional methods, requiring no dissociation or wash steps while still producing robust and reproducible results.

The assay's compatibility with various culture methods and its ability to deliver rapid, reliable data make it a valuable addition to Promega's portfolio of cell health solutions. This portfolio includes: - **CellTiter-Glo Luminescent Cell Viability Assay**: For real-time, non-lytic viability monitoring. - **RealTime-Glo MT Cell Viability Assay**: Also for real-time, non-lytic viability monitoring. - **Caspase-Glo 3/7 Assay**: For apoptosis detection.

These tools, along with the new Lumit assay, provide researchers with a comprehensive suite of assays for measuring specific mechanisms of cell death, inflammation, cytochrome P450 activity, oxidative stress, and autophagy.

The Lumit Cell Proliferation Assay (Human Ki-67) is now available from Promega UK, offering researchers a more efficient and reliable method for assessing cell proliferation.

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